It was well known that B. pertussis cells possess protective antigen, histamine sensitizing factor, heat stable and labile toxin, hemagglutinin, agglutinogen and the others. Previous reports involving above antigenic properties of B. pertussis have been carried out for several years. However, leucocytes promoting property was not yet investigated.
In this report, the results of studies on the leucocytosis, particularly the lymphocytosis, produced in mice by injecting pertussis vaccine were presented.
Especially leucocytes promoting property and histamine sensitizing property of B. pertussis vaccine treated at various temperatures were compared.
The relationship between the leucocytes promoting property and histamine sensitizing property was investigated.
Results were as follows.
1. Although leucocytosis was significantly rised in both 0.5m1. injection and 0.lml injection of pertussis vaccine than in control, at the higher dose (0.5m1 injection) an elevation in white cell count was more significant. The leucocytes response to pertussis vaccine was greater following 0.5 ml injection than following 0.1 ml injection.
2. Lymphocytosis was significantly rised in both 0.5ml injection and 0.lmi in ;action of pertussis vaccine than in control. At higher dose (0.5ml injection), an elevation in lymphocyte count was more significant.
3. Order of elevation in differential leucocytes counts was lymphocyte, polymorph nuclear leucocytes and monocyte.
4. The leucocyte response to pertussis vaccine was 2 fold greater following intravenous injection than following subcutaneous injection.
5. Decrease leucocyte promoting activity and histamine sensitizing activity resulted from exposure to temperature above 561C. Histamine sensitizing activity of pertussis vaccine treated at various temperatures paralleled leucocyte promoting activity.
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